Representative histograms are shown in ( b ) with the quantification of mean intensity in ( c - e ). Flow cytometry analysis of ICAM-1, VCAM-1 and P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1beta treatment. ( b - e ) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin and PECAM-1 with DAPI labelling of the nuclei. ( a ) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. Figure 3 Loss of endothelial SENP1 inhibits EC activation.
